BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. NOTE: This update does not impact the BD Accuri™ C6 Plus. So the choice should be addressed by the kind of flow experiments you plan to do. Our neuroimmunology lab currently has a BD Accuri C6 that has broken about every year and a half since it’s purchase (about 5 yrs old). BD Accuri® - Guide to the FCS Express™ BD Accuri CFlow® Importer Instruction Manual Subject: The FCS Express BD Accuri CFlow Importer, available in FCS Express version 4, allows you to automatically recreate any analysis scheme from BD Accuri CFlow® or BD Accuri CFlow Plus software in FCS Express. To download BD ACCURI C6 MANUAL… 9. BD Accuri™ C6 Plus Flow Cytometer Optical Filter Guide Recommended optical choices for common fluorochromes/dyes Fluorochromes/Dyes Laser Standard Filters Detector Optional Filters2 FITC, BD Horizon Brilliant™ Blue 515, Alexa Fluor® 488, CFSE 488 nm 533/30 FL1 — GFP3 488 nm 533/30 FL1 510/15 JC-1, Fluo-4 AM, SYBR® Green 488 nm 533/30 FL1 — YFP3 488 nm 533/30 FL1 540/20 PE, JC … NOTE: This update does not impact the BD Accuri™ C6 Plus. • For Information On Using BD Accuri C6 Software, See The BD Accuri C6 Software User Guide. There are two ways to make the identification: 1. Select Instrument > Calibrate Fluidics. BD Accuri C6 Flow Cytometer Instrument Manual BD Accuri C6 Software will trigger the notification based on the volume of sheath run through the system or on the date since maintenance was last performed. Analysis of immuno-oncology biomarkers using personal flow cytometry; Assessing NK cell cytotoxicity against tumor cells using personal flow cytometry; Automating dose-response cell cycle analysis with the BD CSampler™ Plus 3 See the BD Life Sciences technical bulletin Optical filter guidelines for fluorescent protein analysis with the BD Accuri™ C6 flow cytometer (applies to both the BD Accuri™ C6 and C6 Plus). Core Size/Flow Rate Combinations.....79 Figure C-1. Default cytometer configuration for the Accuri C6 analyzer: For more information click the following links: C6 cytometer user manual. To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. This portable and compact system holds the analytical power of a cytometer twice its size. To perform compensation, 1. Proper Attachment Of Fluidics Lines, Page 15: Figure 3-8. "WW�oÇ[�rs;$?�N8�)�#Nrb4'����ɻ����!�M�#���̹\W�_>u;t;?�x A Guide to Absolute Counting Using the BD Accuri® C6 Flow Cytometer BD Biosciences August 2011 Page 3 5. Hold the BD CSampler module with both hands and locate the tab in the slot on the back of the BD Accuri C6 (Figure 5). Software options … Windows Security Window. For example, Figure 5 shows a 0.5-µm particle acquired on a BD Accuri C6 using an FSC trigger and threshold of 1,000. BD Accuri™ C6 Plus Filter Guide Product Information Sheets. We have got basic to find a instructions with no digging. <> There are clearly two populations, and yet only one type of particle was run. The DNA histograms produced by the BD Accuri C6 display excellent peak CVs, and this instrument, uniquely, can provide simultaneous linear estimates of nuclear DNA content spanning at least two orders of magnitude (a range of 0.34 to 80.9 pg). All current service contracts will be honored. Figure 6 shows how the FCS file data will look when initially opened in FlowJo. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. Connect the cable to the socket at the back of the BD Accuri C6 and switch on the BD Accuri C6. Even new flow cytometry users find it so easy to learn and use that Sliding Luer Lock Fittings Off Of Pump Head, Page 25: Troubleshooting And Technical Support, Page 26: Figure 5-1. User Tracking Installer Icon .....80 Figure C … Only operate the Accuri C6 Flow Cytometer with an original copyrighted version of software authorized by Accuri. endobj If you can simplify cell analysis ITC Data Analysis in versions 8. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. We have got basic to find a instructions with no digging. On the Accuri C6, all compensation is performed manually. Comprehending as competently as deal even more than additional will find the money for each success. Turn on the computer and log in with your UCF info. Flow cytometry should be easy. DI water Daily (if the cytometer is not shut down) Run the decontamination and cleaning cycles. ���j��]o��P�Pwʄ�> ^��-3���˼�/�~Ϧ>4��bUTB G�%*��I��%Rh�"%�E �ѡ�*���g����π%��f�7�æ����.�J����Z:_,H�O<=/�#��gT��v_hǒ)pb�[�ڗ�R�5��mӶ�;�в���߷~�S����j�I��^�9d�8�G�{f�4"�Y�a4���Y��%�k���. Within 5 minutes of completing the water run, place a calibration sample on the SIP. BD Accuri C6 with plate handler April 2016 This protocol contains instructions for setting up an Accuri C6 with plate handler. Direct counts on the BD Accuri C6 Plus correlate highly with counting beads and are more precise than manual counts. Install BD CSampler software. To install BD Accuri C6 Software, run BDAccuriC6SoftwareInstaller.exe. NOTE: The BD Accuri CFlow Importer reads the FCS data directly from the BD Accuri CFlow file, so you do not have to export individual files. Breakthrough with the BD Accuri™ C6 Plus Cell Analyzer. This note discusses some of the basic concepts needed to perform small particle acquisition and analysis properly. Peristaltic Pumps And Luer Locks, Page 24: Figure 4-6. Install, update, and run anti-virus protection software on a regular basis. Start the Accuri CSampler software. Download: Bd Accuri Analysis Manual Printable 2019 Online Reading At BRAZILFILMFESTIVAL.INFO Free Download Books Bd Accuri Analysis Manual Printable 2019 We All Know That Reading Bd Accuri Analysis Manual Printable 2019 Is Beneficial, Because We Can Easily Get A Lot Of Information From Your Resources. x��XKo�H�#��� M��^EH �D�H���=x�!삙�1Q��V� ��m8ەz~]U]ݤ? BD Accuri C6 Software will display this notification based on whichever trigger is reached first. BD Accuri C6 flow cytometer for the analysis of plant genome sizes. Core Size/Flow Rate Combinations.....79 Figure C-1. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project: flowcyt@illinois.edu, (217) 244-0559. BD Accuri C6 Flow Cytometer Instrument Manual 7820018-01 Rev-2 1 The BD Accuri™ C6 flow cytometer offers an entirely new vision for the role of flow cytometry in life science research. 3. (In this example, the file is FCS Express Import Demo.c6.) For detailed procedures, see the BD Accuri C6 Flow Cytometer Instrument Manual. Cited in over 9,000 publications, the BD Accuri C6 Plus enables you to go beyond your current research potential. 3 0 obj BD Accuri C6 Software User Guide - Manual. Along with traditional forward and side scatter, the C6 is an ideal instrument for basic analytical cytometry. �&�Yl�H�$��9It��$�%�|X��̒��L���B��D��kt��/��#_� )a!�X܌��N�Xb��CF���D'�)A�K�ȃq��j���T����L�QO��< ��a�� Use another characteristic of the particle, such as fluorescence, to BD Accuri® C6 flow cytometer, certain items must be considered to ensure proper data acquisition. The BD CSampler will auto- matically align itself and then remain in the home position (Figure 8 - 9). Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. Without the BD CSampler installation manual online for full details. 10. %PDF-1.5 Scheduling. Nos. Frequency Task Supplies and parts After every experiment Run filtered DI water at the Fast rate for 3 to 5 minutes to prevent clogging. To change a parameter: Science is hard. Flow Cytometry Within Reach The analytical power and versatility of today’s laser-based flow cytometry systems Systems has broken about every year and for core immunophenotyping studies and services from each plot. Print version: Text Size: Related Files » C6 manual » BC aquatic uses ... Accuri C6 consumables Consumables (fluids): (MSDS linked at the right -->) Cleaning solution, KR-225 (citric acid). 8. Install, update, and run anti-virus protection software on a regular basis. Please complete the following information to request a demonstration of the BD Accuri™ C6 flow cytometer. 11. And also by the ability to access our manual online or by storing it on your desktop, you have convenient answers with BD ACCURI C6 MANUAL. 3 VALIDATING THE PERFORMANCE OF THE BD ACCURI C6 FLOW CYTOMETER .....11 3.1 Running Validation Beads (Daily Start-Up) .....11 3.2 Saving Validation Bead Data .....15 3.3 Analyzing and Recording Validation Bead Data.....15 3.4 Monitoring Validation Bead Data.....19 3.5 Troubleshooting Validation .....20 4 MANUAL DATA ACQUISITION.....22 4.1 Viewing the Manual Collect Tab.....22 4.2 … endobj 2 0 obj stream • Calibrationshould be performedin the same tube type as the experimental sample. BD CSampler Software User Guide iv 7820099-01 Rev-2 TABLE OF FIGURES Figure 1-1. BD Accuri C6 with single tube loader protocol This protocol contains instructions for setting up an Accuri C6 with Tube Loader. BD has discontinued selling the BD Accuri™ C6 flow cytometer as of December 31, 2016. A broad dynamic range of detection makes it easy to analyze cells as varied in size as platelets and eosinophils in the same data file. BD ACCURI C6 MANUAL might not make exciting reading, but BD ACCURI C6 MANUAL comes complete with valuable specification, instructions, information and warnings. Place the mat in position (Figure 10). The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you Post-acquisition compensation can be performed using Accuri C6 software Page 2/13. Of the BD Accuri C6 Flow Cytometer Instrument Manual Research Use Only. *Install the BD Accuri C6 Software. 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